Services

Human TCRß Profiling - TCRsafe®

HSDiagnomics provides a complex service to customers, starting normally from genomic DNA extracted from blood, tissues, or FFPE samples. Using our proprietary primer sets covering all 43 V-segments and 14 J-segments for the T-cell Receptor ß (TCRß) CDR3 locus, plus an inbuilt contamination protection system, we amplify the T-cell repertoire and perform a deep sequencing step: Typically 1-2 million read-pairs per sample are generated.

In the 2nd part of the TCRsafe®-service, with an in-house developed software package HSDiagnomics processes and assembles raw reads into clusters and assigns V/J-segment IDs and CDR3 DNA and amino acid sequences. The result of our analysis sent out to customers is a table of clusters and frequencies, i.e. a high-resolution profile of TCRß.

At a glance, our TCRsafe® service comprises the following steps:

  • 2-step multiplex PCR with contamination protection
  • Optimized primer concentrations to ensure quantitative analysis
  • Generation of barcoded sequencing libraries
  • Ultra-deep sequencing (HiSEQ, MiSEQ) with typically 1-2 million read-pairs per sample
  • Highly-sensitive clustering of reads
  • Assignment of V/J-segment IDs according to the IMGT annotation system
  • Generation of comprehensive TCRß frequency tables (spreadsheets), see an example here
  • Customized bioinformatic analyses

Mouse TCRß Profiling - TCRsafe®

There are strong interests by researchers in academic and industry labs to perform analysis of T-cell response under various treatments in the mouse model system.

HSDiagnomics addresses these needs by a proprietary mouse TCRsafe® analysis. It is a thoroughly optimized quantitative TCRß assay, equipped with the same contamination protection system as the human TCRsafe® kit. Besides using fresh tissue, blood, and cultured cells, the assay can be also performed on formalin-fixed and paraffin-embedded tissues.

Examples for TCRß analyses of 5 wildtype (C57Bl/6) mice thymi are given here and the optimization of the quantitative TCRß analysis employing an synthetic reference template has been published here.

Interested customers are welcome to contact us for more details.

TCRsafe® – Fee-for-Service Tasks and Research Collaboration Projects

HSDiagnomics has processed several hundreds of samples using its TCRsafe® technology in fee-for service tasks and research collaboration projects carried out with partners both in academia and industry.

As a service provider and partner we can offer our well tested technology for generating, handling and analyzing huge data volumes. We guarantee fast delivery of results and offer systematic tools for displaying and storing results in a user-friendly way.

The following features will enable robust and relevant data acquisition in your project in a very short period of time:

  • Data quality and safety: Contamination protection will be active from project start to end.
  • Data mining packages by HSDiagnomics will help you to find the relevant TCRs and common TCR motifs in large sample cohorts – easy and precise.
  • Specific questions and needs of customer projects can be addressed by customized software modules.
  • HSDiagnomics will organize your data storage.

We are happy to discuss all relevant details with you – just give us a call!

The infographic depicts customer-provided items on the left, including tumor tissue, normal tissue, lymphocytes, and blood. The services offered by HSDiagnomics are listed on the right. These include tissue preparation, FACS isolation of T-cell subsets, TCRsafe®: quantitative TCRa/β discovery, TCR α/β pairing, identification of tumor-specific (ts) TCRs, prioritization of promising immunodominant (ts) TCRs using proprietary selection criteria, TCR ectopic expression and testing, and counseling services.

 

HS Diagnomics GmbH

Schloßstrasse 110
12163 Berlin
Germany

Tel. +49(30)700 14 53 30
Fax +49(30)546 11 698

Customer Support
Tel. +49(30)700 14 53 31

HSDiagnomics

News

06/2024
Project Update: HS Diagnomics has entered into the international collaborative project ELEVATE with TU Wien, NMI Tübingen, Sciospec, UpNano, and THT Biomaterials
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10/2023
HSDiagnomics partners with Charité University Medicine Berlin, TheryCell GmbH, and EPO GmbH in an innovative project to advance TCR-T therapy for solid tumors
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06/2020
TCRsafe® analysis of a Runx1 knockout mouse model provides evidence for a role of RUNX1 as a recombinase cofactor for TCRß rearrangements and pathological deletions
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08/2019
ZIM-Project TCR-specific Antigen Identification - TAgID
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04/2018
Patent granted for the identification of tumor-specific T-cell receptors
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