Innovations
Granted Patents
Innovation TCRsafe®
For an accurate analysis of the T-cell receptor (TCR) repertoire, HSD’s proprietary TCRsafe®-method is based on the granted patent “Methods and primer sets for high throughput PCR sequencing” (EP2746405B1). The technology featuring an intrinsic contamination protection system effectively prevents cross-sample-contaminations, which is of great advantage considering the distinct sensitivity of current next generation sequencing (NGS) technologies. Furthermore, employing a synthetic TCRß reference template, all primers were carefully titrated, to allow even amplification rates for all possible V/J-combinations. The method is applied to the balanced high throughput amplification and sequencing of TCR repertoires. E.g. of tumor-infiltrating lymphocytes (TIL), corresponding adjacent normal tissue and/or autologous peripheral blood cells. This technology is a prerequisite for the identification of dominant, tumor-specific T cells and their TCRs and applied to lung cancer, breast cancer, ovarian cancer, prostate cancer, melanoma specimens, etc.
Innovation Method for providing tumor-specific T cells
HSD’s invention “Method for providing tumor-specific T cells” (EP3180433B1) encompasses the quantitative TCRsafe® high throughput TCR repertoire analysis of TIL compared to lymphocytes infiltrating adjacent corresponding normal tissue (and/ or present in peripheral blood) and the in-house TCR integrate software, which enables the identification of the tumor-specific T cell receptor (TCR) repertoire for each individual patient. Complete decoding of paired α and ß chains of tumor-specific TCRs facilitates synthesis and cloning of TCRs that can be applied to genetic engineering of patients’ autologous T cells. These engineered T cells are used as tumor-specific probes for the discovery of tumor antigens. They can also be used for adoptive T cell therapy (see home page TheryCell GmbH https://www.therycell.de).
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News
06/2024
Project Update: HS Diagnomics has entered into the international collaborative project ELEVATE with TU Wien, NMI Tübingen, Sciospec, UpNano, and THT Biomaterials
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10/2023
HSDiagnomics partners with Charité University Medicine Berlin, TheryCell GmbH, and EPO GmbH in an innovative project to advance TCR-T therapy for solid tumors
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06/2020
TCRsafe® analysis of a Runx1 knockout mouse model provides evidence for a role of RUNX1 as a recombinase cofactor for TCRß rearrangements and pathological deletions
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08/2019
ZIM-Project TCR-specific Antigen Identification - TAgID
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04/2018
Patent granted for the identification of tumor-specific T-cell receptors
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